The Immunological Synapse Part C (Methods in Cell Biology)
Clément Thomas, Lorenzo Galluzzi, 9780443218682, 978-0443218682, 978-0-443-21868-2, B0DK9K428G, 9780443218699, 978-0443218699, 0443218684, 0443218692
The Immunological Synapse - Part C, Volume 193 in the Methods in Cell Biology series provides state-of-the-art and detailed methods for the study of the T cell, natural killer (NK) cell and B cell immunological synapses. Topics covered in this third volume include tau-STED microscopy for 3D-quantitative colocalization of lytic granule markers, image processing approaches for 3D-analysis of markers and for microtubule remodeling quantification at the immunological synapse, flow cytometry to analyze NK cell-target cell conjugation, imaging flow cytometry to quantify a range of different subcellular processes at the immunological synapse, imaging techniques aimed at quantitatively analyzing B cell immunological synapse formation, quantification of force-mediated antigen extraction in the B cell immune synapse using DNA-based tension sensors, measuring interaction forces between T lymphocytes and their target cells using live microscopy and laminar shear flow chambers, measuring interaction kinetics between T cells and their target tumor cells with optical tweezers, and gauging antigen recognition by human primary T-cells featuring orthotopically exchanged TCRs of choice. Additionally, this volume includes methods to isolate and characterize primary NK cells and enrich the KIR2DL1+ population, and to expand γδ T cells and evaluate their cytotoxicity.
- Covers various methods related to the study of the immunological synapse
- Includes detailed, point-by-point, methods as well as various important notes
- Provides the authority and expertise from an international board of leading scientists